Detailed view for PF3D7_1210900

Basic information

TDR Targets ID: 5156
Plasmodium falciparum, GPI mannosyltransferase 1
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.5517 | Length (AA): 441 | MW (Da): 53327 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 11

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05007   Mannosyltransferase (PIG-M)

Gene Ontology

Mouse over links to read term descriptions.
GO:0016758   transferase activity, transferring hexosyl groups  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005789   endoplasmic reticulum membrane  
GO:0005783   endoplasmic reticulum  
GO:0000030   mannosyltransferase activity  
GO:0006506   GPI anchor biosynthetic process  
GO:0006486   protein amino acid glycosylation  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
87 409 2a06 (C) 25 359 16.00 0.00000076 0 0.8 -0.91
191 272 4lua (A) 55 138 30.00 0.66 0.37 0.292041 1.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile merozoite, sporozoite, early schizont, late schizont, late trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, early trophozoite, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 24 hs, Oocyst, Sporozoite, Female Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Ring. Otto TD Zanghi G
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128297)

Species Accession Gene Product
Arabidopsis thaliana AT5G22130   GPI mannosyltransferase 1
Babesia bovis BBOV_II000550   mannosyltransferase (PIG-M) containing domain
Brugia malayi Bm1_10780   mannosyltransferase
Candida albicans CaO19.12050   similar to human angiotensin II type 1b receptor
Candida albicans CaO19.4581   similar to human angiotensin II type 1b receptor
Caenorhabditis elegans CELE_B0491.1   Protein B0491.1
Cryptosporidium hominis Chro.10419   mannosyltransferase-related
Cryptosporidium parvum cgd1_3720   PIG-M mannosyltransferase,8 transmembrane domain
Dictyostelium discoideum DDB_G0288899   DXD motif-containing protein
Drosophila melanogaster Dmel_CG9865   CG9865 gene product from transcript CG9865-RA
Echinococcus granulosus EgrG_000822400   Mannosyltransferase family protein
Entamoeba histolytica EHI_103330   mannosyltransferase, putative
Entamoeba histolytica EHI_029580   mannosyltransferase, putative
Echinococcus multilocularis EmuJ_000822400   Mannosyltransferase family protein
Giardia lamblia GL50803_17502   Mannosyltransferase
Homo sapiens ENSG00000143315   phosphatidylinositol glycan anchor biosynthesis, class M
Leishmania braziliensis LbrM.30.1970   mannosyltransferase, putative
Leishmania donovani LdBPK_302030.1   mannosyltransferase, putative
Leishmania infantum LinJ.30.2030   mannosyltransferase, putative
Leishmania major LmjF.30.2030   mannosyltransferase, putative
Leishmania mexicana LmxM.29.2030   mannosyltransferase, putative
Loa Loa (eye worm) LOAG_02158   mannosyltransferase
Loa Loa (eye worm) LOAG_11656   hypothetical protein
Mus musculus ENSMUSG00000050229   phosphatidylinositol glycan anchor biosynthesis, class M
Neospora caninum NCLIV_054210   GPI mannosyltransferase 1, putative
Oryza sativa 4333686   Os03g0670200
Onchocerca volvulus OVOC11090   GPI mannosyltransferase 1 homolog
Plasmodium berghei PBANKA_0609300   GPI mannosyltransferase 1, putative
Plasmodium falciparum PF3D7_1210900   GPI mannosyltransferase 1
Plasmodium knowlesi PKNH_1311000   GPI mannosyltransferase 1, putative
Plasmodium vivax PVX_084575   GPI mannosyltransferase I, putative
Plasmodium yoelii PY02427   mannosyltransferase-related
Saccharomyces cerevisiae YJR013W   Gpi14p
Schistosoma japonicum Sjp_0116980   ko:K05284 phosphatidylinositol glycan, class M, putative
Schistosoma japonicum Sjp_0093010   hypothetical protein
Schistosoma mansoni Smp_128810   hypothetical protein
Schmidtea mediterranea mk4.002703.03  
Trypanosoma brucei gambiense Tbg972.6.3090   mannosyltransferase, putative
Trypanosoma brucei Tb927.6.3300   GPI alpha-mannosyltransferase I
Trypanosoma congolense TcIL3000_6_2850   mannosyltransferase, putative
Trypanosoma congolense TcIL3000_6_2840   GPI transamidase subunit PIG-U, putative
Trypanosoma cruzi TcCLB.511507.50   mannosyltransferase, putative
Trypanosoma cruzi TcCLB.503909.100   mannosyltransferase, putative
Toxoplasma gondii TGME49_310100   mannosyltransferase (pig-m) protein
Theileria parva TP04_0525   mannosyltransferase, putative

Essentiality

PF3D7_1210900 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.3300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.3300 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.3300 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.3300 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YJR013W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0609300 Plasmodium berghei Essential plasmo
TGME49_310100 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

17 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1210900 (Plasmodium falciparum), GPI mannosyltransferase 1
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